Fig 1.

Exposure of minor capsid proteins VP2/3 within the ER. CV-1 cells were infected with SV40, fixed in cold methanol, and then stained for VP2/3 (FITC) and ER marker PDI (Cy3). (A) A 3-h sample. No green VP2/3 stain can be seen, while the PDI stain is seen in red. The nucleus is highlighted by the blue DAPI stain. (B) Sample fixed at 6 h. The top two panels show a punctate VP2/3 stain, seen in green, and the PDI stain, seen in red. The bottom two panels show a 5-fold magnification of the VP2/3 stain and of a merge image. The yellow VP2/3 stain in the 5× merge indicates the colocalization of VP2/3 with the ER marker. (C) Sample fixed at 12 h. Note that the VP2/3 stain in the merge is green. Compare this image to panel B. The scale bars for the three images in panel A, the top two images in panel B, and the three images in panel C correspond to 10 μm.