Fig 2.

Localization of the VP2/3 immunostain over time. Experiments were carried out in triplicate, in which a total of 100 × 3 cells, immunostained for VP2/3, were examined at each time point. The average numbers of cells in which the VP2/3 stain colocalized or did not colocalize with the PDI immunostain at each time are indicated. Note that some cells contained VP2/3 associated with PDI and also VP2/3 not associated with PDI. These cells were included in each of the columns at their respective time points. No VP2/3 immunostain was detected at 3 hpi.