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BrdU-labeled SV40 genomes are first detected outside of the ER. CV-1 cells were infected with BrdU-labeled SV40 and fixed in cold 100% methanol at 8 and 10 hpi. Cells were treated with DNase for 10 min and immunostained for BrdU (Cy3) and ER marker PDI (FITC). A representative merge image is shown for each time point. Scale bar, 10 μm.
