Fig 4.

Colocalization of viral proteins in virus-infected cells. A549 cells were infected with the indicated viruses at an MOI of 5. At 12 h postinfection, cells were fixed and incubated with an anti-NP (A, G, M, m, S, and s), anti-HA (B, H, N, n, T, and t), or anti-M1 (C, I, O, o, U, and u) monoclonal antibody. The nucleus was counterstained with Hoechst 33258. D, J, P, p, V, and v, merged images for HA and M1; E, K, Q, q, W, and w, merged images for NP and M1; F, L, R, r, X, and x, merged images for NP, HA, and M1.