Fig 6.

AnxA6 colocalizes with M2 at the plasma membrane of virus-infected human cells. A431 cells were transfected with a plasmid encoding the AnxA6-GFP fusion protein and then infected 24 h later with influenza A/WSN/33 virus at an MOI of 5. After fixation at 14 h p.i., cells were stained with a mouse anti-M2 MAb followed by Alexa Fluor 555-conjugated goat anti-mouse IgG. Single optical sections (upper panels) and representative z-plane reconstructions (lower panels) are shown. The inset in panel d shows a magnified (×3.7) region at the plasma membrane. Arrows indicate colocalization of AnxA6 and M2 at the plasma membrane. Bar, 10 μm.