Fig 10.

The insulator protein CTCF binds to a region around the origin of lytic replication in a primary MDV lymphoma, PBL, and the 265L cell line. (A) Chromatin immunoprecipitation was carried out using an antibody against CTCF and was quantified using qPCR. Binding was strongest at the OriLyt. (B, C, and D) Similar ChIP assays were then carried out using antibodies against the cohesion factors Rad21, SMC1, and SMC3. Results are shown as percentages of total input. All three molecules were found bound at the same region as CTCF. A site 3 kb upstream of OriLyt showed no detectable binding for CTCF, Rad21, SMC1, or SMC3. Binding of these factors was not detected consistently in 265L cells (data not shown).