Fig 4.

Histone modifications and binding of RNA Pol II in primary MDV lymphoma and PBL. Chromatin immunoprecipitation was carried out with antibodies against histone modifications and RNA Pol II phosphorylated at serine 5 (pS5) on chromatin extracted from a primary MDV lymphoma. (A) H3K27me3; (B) H3K9me3; (C) H3K4me3; (D) H3K9Ac; (E) RNA Pol II pS5. Results are shown as percentages of total input. The chromatin profile and regions of active transcription were very similar to those seen for the tumor-derived cell lines. (F) Map of the region and locations of qPCR primers (triangles).