Fig 7.
CpG methylation of a gene (UL30) in the UL region of the MDV genome. Methylated DNA precipitation was carried out on fragmented DNAs from PBL isolated from two RB1B-infected line P chickens that developed lymphomas, from a primary MDV lymphoma isolated from an RB1B-infected line P chicken, and from two T-cell lines (265L and RPL1). Results are shown as percentages of total input. Methylation within the ICP4 gene was compared to methylation within the intragenic region of UL30. There was no detectable methylation in the UL30 gene within the PBL and tumor, but both cell lines had detectable methylation.