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Initiation of MDV lytic gene expression by chromatin-modifying drugs. Western blotting was carried out on the 265L cell line treated with AZA, TSA, and NaB. Samples were taken 0, 24, and 48 h after drug treatment, separated by SDS-PAGE, Western blotted, and probed with antibodies against γ-tubulin and pp38. All three treatments induced the expression of pp38, a lytic MDV antigen.
