Table 1.
Species B serotype-mediated eGFP transgene expression in different CHO-CD46-expressing cells compared to parental CHO and human A549 cells
| eGFP transgene | eGFP expression level for cell line |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Fold change compared to CHOa |
% of A549 expressionb |
No. of vp/cell needed to reach MFI of 100c |
|||||||||
| CHO-CD46#6 | CHO-CD46#1 | CHO-CD46#2 | CHO | CHO-CD46#6 | CHO-CD46#1 | CHO-CD46#2 | CHO-CD46#6 | CHO-CD46#1 | CHO-CD46#2 | A549 | |
| Ad3-eGFP | 18 | 55 | 192 | 0.24 | 19 | 60 | 211 | 8,207 (27) | 2,597 (16) | 717 (4.2) | 1,513 (2.7) |
| Ad7-eGFP | 37 | 85 | 354 | 0.23 | 21 | 50 | 202 | 2,919 (9.5) | 1,210 (7.3) | 267 (1.57) | 602 (1.1) |
| Ad11-eGFP | 40 | 88 | 79 | 1.03 | 49 | 105 | 95 | 634 (2.1) | 291 (1.8) | 314 (1.8) | 329 (0.6) |
| Ad35-eGFP | 60 | 106 | 96 | 2.66 | 177 | 305 | 284 | 307 | 165 | 170 | 562 |
a
Ratios of eGFP mean fluorescence intensity levels from CD46-expressing cell line to that from parental CHO cells were determined for a 1,000-vp/cell input.
b
eGFP mean fluorescence intensity levels of CD46-expressing cells as percentages of those of A549 cells were determined for a 1,000-vp/cell input.
c
Regression lines were calculated for the eGFP expression levels; numbers in parentheses correspond to fold virus concentration input over that of Ad35-eGFP.