Fig 4.

SMAD5 was downregulated by miR-K12-11 in de novo KSHV-infected HEK293T cells. (A) The expression levels of miR-K155 or miR-K12-11 were detected 72 h after rKSHV.219 infection (MOI = 10) in HEK293T cells and control cells. (B and C) SMAD5 expression was downregulated in response to rKSHV.219 de novo infection. Western blotting and qRT-PCR were used to detect SMAD5 expression at the protein and mRNA levels, respectively. Densitometry values represent the percentage of SMAD5 normalized against β-actin compared with control cells. (D) Sponge/K12-11 can rescue SMAD5 expression in HEK293T/219 cells. HEK293T/219 cells were transduced with a lentiviral miR-K12-11-specific sponge inhibitor or a control vector, and then the expression levels of SMAD5 and β-actin were determined by Western blotting.