Fig 5.

SMAD5 was significantly underexpressed in KSHV-positive PEL cells. (A) miR-K155 or miR-K12-11 expression levels were determined in the indicated cells by bulge-loop qRT-PCR. The percentage of SMAD5 normalized against β-actin and compared with the untreated control is shown. (B) The mRNA levels of SMAD5 in the indicated cells were detected by qRT-PCR. (C) Western blotting was used to monitor the expression levels of SMAD1/2/3/5 in KSHV-positive cells in contrast to KSHV-negative cells. Densitometry values denote the percentage of SMAD5 normalized against β-actin and compared with Ramos cells. (D) Sponge/K12-11 rescued SMAD5 expression in BCBL1 or BC3 cells.