Table 1.
Sequences of primers used for PCR and qRT-PCR
| Name | Sequencea |
|---|---|
| SMAD1UTR-256KpnIF | TTTggtaccGCAGGACTTTGTGTACAG |
| SMAD1UTR-563XhoIR | TCCctcgagCAGCAAGTATGGTCAGCAT |
| SMAD2UTR-8050KpnIF | TGCggtaccAGCCAGAATGTGTTGTTAAC |
| SMAD2UTR-8700XhoIR | AACctcgagCATGGTAAACAACTCAAATGG |
| SMAD3UTR-4168KpnIF | TTTggtaccCAGCATCCAGAAACACCAAAC |
| SMAD3UTR-4388XhoIR | TCCctcgagACACATCAGAGTCCAGAACAG |
| SMAD5UTR-580KpnIF | TGAggtaccACTATGCACTGCTGTAACTGG |
| SMAD5UTR-930XhoIR | AAGctcgagAGGACGTAAGGATAAGCATC |
| SMAD5UTR-MRE1F | CAAGTATATGTCATTTACTCAAAATCTGTCATAAcgtaatCTTTATAGCTAGTGACAG |
| SMAD5UTR-MRE1R | CTGTCACTAGCTATAAAGattacgTTATGACAGATTTTGAGTAAATGACATATACTTG |
| SMAD5UTR-MRE2F | GACAATGTTGCAAGAACTCTATTTTTGACATGgtaattTCTTTTATTTTGCACTTTTAT |
| SMAD5utr-MRE2R | ATAAAAGTGCAAAATAAAAGAaattacCATGTCAAAAATAGAGTTCTTGCAACATTGTC |
| Tgfbr2-1EcoRIF | AATgaattcGCCACCATGGGTCGGGGGCTGCTCAG |
| Tgfbr2-1704BamHIR | AATggatccCTATTTGGTAGTGTTTAGGGAG |
| SMAD5qPCR-F | CCAGCAGCTGCAGCCTCAAAAT |
| SMAD5qPCR-R | TGCCGGTGATATTCTGCTCCCCAA |
| SMAD5-1SalIF | CGCgtcgacCATGACGTCAATGGCCAGCTT |
| SMAD5-1395NotIR | CGCgcggccgcCTTATGAAACAGAAGATATGG |
a
Lowercase nucleotides represent restriction enzyme recognition or PCR-based mutagenesis sites.