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(a) Fluorescence spectra of the aqueous solution of Rh800-S[8] complex upon adding ACh. [Rh800] = 40 nM, [S[8]]/[Rh800] = 800, and [ACh]: a) 0 mM, b) 1.1 mM, c) 3.4 mM, d) 7.7 mM, e)12 mM, and f) 16 mM. Inset shows concentration dependence of ACh on the fluorescence intensity of Rh800. (b) Plots of f versus [ACh] and f versus [ACh]2.
