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Fluorescence changes (ΔF at 710 nm) in Rh800-S[8] solution upon adding ACh, other neurotransmitters (dopamine, GABA, glysine, l-asparatic acid), basic amino acids (l-histidine, l-lysine), ammonium chloride, and choline. 100 μL of these chemicals (350 mM in water) was added to the Rh800-S[8] solution (3 mL), where [Rh800] = 40 nM and [S[8]]/[Rh800] = 800.
