Figure 3. Changes in axonal transport after nerve-crush injury.

(A) Kymographs from example movies of ANF-GFP labeled vesicles in an uninjured axon (Movie S3), the distal stump 3 hours after injury (Movie S4) and the proximal stump (Movie S5) 3 hours after injury. (B) Particle density (anterograde, retrograde, and stationary) was quantified per 100 µm of axon length. In the proximal stump 3 hours after injury, there was a 90% increase in anterograde particle density (p-value = 0.03, n = 16), but no significant change in retrograde particle density (p-value<0.01, n = 16). In contrast, transport was almost completely halted in the distal stump (p value<0.01, n = 8). Error bars represent standard error of the mean.