Figure 7. The early arrested phenotype is not due to Wolbachia.

Eggs at different times AED were fixed and incubated with antibodies against β-tubulin (green), acetylated-α-tubulin (blue), and counterstained with Hoechst 33258 (red). Unfertilized eggs showed cytoplasmic asters that increase in number with age (A,B,C, arrows); old eggs also display smaller cortical asters (arrowheads). (E) Occasionally a sperm tail is found within the eggs that show cytoplasmic asters (arrowheads). Bacteria are associated with the cytoplasmic arrays of microtubules (arrowheads, inset D). (F) Unfertilized eggs from the uninfected KOS1 females also contain cytoplasmic asters. Bacteria are also observed at the poles of the meiotic-like spindles (arrowheads, F,G), where aster-like microtubule arrays are found (arrows, F′,G′). Scale bar is 25 µm in A,B,E; 15 µm in C; 4 µm in D; 5 µm in F,G.