Figure 7. Short-term plasticity at Cortico-LA synapses is perturbed in absence of αGDI.

(A) Iterative stimulations (20 Hz/4 sec) were applied at Cortico-LA synapses in Gdi1 WT and Gdi1-null mice. Scale bars: 50 (up) and 100 (bottom) pA and 250 msec. (B) The pronounced synaptic depression in Gdi1-null mice is well illustrated by the extraction of the last EPSCs of the train response. Scale bars: 50 (up) and 100 (bottom) pA and 10 msec. (C) Mean EPSC amplitude at a given stimulation for each genotype. (D) Cumulative plot based on the same data, allowing to visualize the deficit in refilling rate at Gdi1-null synapses (see material and methods section for further details). (E₁) Ready releasable pool size (RRP) and refilling rate (E₂) were calculated in Gdi1-null and Gdi1 WT preparations and expressed as % of initial EPSC size. Number of recorded cells is indicated. **p<0,01.