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. 2012 Jan 23;7(1):e30139. doi: 10.1371/journal.pone.0030139

Figure 3. CD40-B cells directly present Ag to naïve CD8+ T cells.

Figure 3

A. Highly purified sorted CD40-B cells can induce a CD8+ T cell response. Mice were immunized as in Figure 2 with sorted or unsorted CD40-B cells matured with LPS and loaded with OVA. Percentage of effectors (CD8+CD45.2+) generated is shown at d4 post-immunization. B. Direct priming of OVA-specific CD8+ T cells by CD40-B cells. 5×106 CFSE-labelled OT-1 T cells were adoptively transferred into either C57BL/6 or Bm1 recipients followed by immunization on the same day with 6×106 LPS-treated CD40-B cells loaded with OVA (B LPS OVA) or an irrelevant peptide (B LPS) or 2×106 LPS-matured DCs loaded with OVA peptide (DC OVA). The proliferation of OVA-specific T cells was analyzed 48 h later. The histograms show CFSE-profile gated on OVA-specific CD8+ T cells using Kb-OVA tetramer staining. The percentage of undivided cells is indicated on each histogram. 3 independent experiments with 2 mice per group.