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. 2012 Jan 23;7(1):e30102. doi: 10.1371/journal.pone.0030102

Figure 7. Binding analysis of the half-sites of kdpFABCBS.

Figure 7

SE analysis of binding of KdpEDBD to S1 (kdpFABCBS—7) (A)and S2 (kdpFABCBS—1) (B) half-sites revealed a 1∶1 stoichiometry. Mixtures of KdpEDBD and DNA were spun at 9,000 (•), 19,800 (□) and 34,000 (Δ) rpm. The Kds obtained for KdpEDBD binding at half-sites S1 was 350±100 nM and for S2 was 200±100 nM using a one site binding model (AB) in SEDPHAT. The molecular weights calculated from the SE data were 30,000±1,500 for kdpFABCBS—1 and 30,000±2,500 for kdpFABCBS—7.