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. 2012 Jan 23;7(1):e30575. doi: 10.1371/journal.pone.0030575

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Kajiho et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) HeLa cell lysates were immunoprecipitated with normal rat IgG or anti-odin antibody, followed by immunoblotting with antibodies as indicated. (B) FLAG-RIN family or FLAG-mock were transfected into HEK293T cells. Cells lysates were immunoprecipitated with anti-FLAG antibody, followed by immunoblotting with antibodies as indicated. (C) FLAG-RINL and the indicated deletion mutants of myc-odin were transiently transfected into HEK293T cells. Cells lysates were immunoprecipitated with anti-myc antibody, followed by immunoblotting with antibodies as shown. (D) The indicated deletion mutants of FLAG-RINL were transiently transfected into HEK293T cells. Cells lysates were immunoprecipitated with anti-FLAG antibody, followed by immunoblotting with antibodies as indicated. (E) Myc-odin and V5-RINL were co-transfected with FLAG-tagged constitutively active (CA, lanes 2 and 4) or mock (lanes 1 and 3) into HEK293T cells. Cell lysates were immunoprecipitated with anti-myc antibody, followed by immunoblotting with antibodies as indicated. Aliquots of total lysates were also immunoblotted with antibodies as indicated.