Figure 4. LIF neutralizing studies in a fetal lung explant culture system.

(A) Representative examples of lung explants treated daily with normal IgG (control; 1 µg/mL), anti-LIF IgG (1 µg/mL) or FGF-10 (500 ng/mL), after 4 days in culture. Original magnification ×25. (B) Number of total airway buds; (C) Epithelial perimeter; (D) Area; (E) External perimeter of treated lung explants. Inhibition of LIF action significantly stimulated lung branching in a similar way than FGF-10. Results are expressed as D₄/D₀ ratio. p<0.05: ^* vs. control IgG.