Table 4. Microbial colonization of ossicles after HHP treatment (350 MPa, 10 minutes) with and without the addition of antibiotics to the media.
Samplenumber | Species | Semi-quantification after pressure treatment in the absence of antibiotics | Semi-quantification after pressure treatment in the presence of antibiotics |
1 | No bacterial growth | − | − |
2 | No bacterial growth | − | − |
3 | Acinetobacter baumannii | ++ | − |
Leuconostoc mesenteroides ssp. cremoris | − | +/− | |
4 | Staphylococcus hominis | ++ | − |
Micrococcus luteus | +/− | − | |
Staphylococcus auricularis | +/− | − | |
Staphylococcus capitis | +/− | − | |
Staphylococcus epidermidis | ++ | − | |
5 | No bacterial growth | − | − |
6 | No bacterial growth | − | − |
All bacterial strains listed above were detected by conventional culture techniques. A combination of vancomycin (11.1 µg/ml), clindamycin (0.75 µg/ml) and imipenem (3.7 µg/ml) was added to samples 1 to 3. A combination of cefuroxime (11.1 µg/ml), gentamicin (44.4 µg/ml) and imipenem (3.7 µg/ml) was added to samples 4 to 6. See Table 2 for an explanation of the symbols used.