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. 2012 Jan 23;7(1):e30622. doi: 10.1371/journal.pone.0030622

Figure 1. The skp1-a7 mutant shows the bent spindle in MI.

Figure 1

(A) Meiosis and sporulation were induced for wild-type (WT) and the skp1-a7 strains at 33°C, the semi-restrictive temperature. Images of DIC and DNA stained with DAPI are shown. (B) The number of spores in WT and skp1-a7 asci were counted. Percentages of aberrant asci only are shown in the graph. Most of WT cells generated four spores, whereas skp1-a7 cells produced abnormal asci with 1∼3 and ≥5 spores. Meiosis was induced at the indicated temperature. n = 250. (C) Percentages of aberrant ‘crossing’ spindles in MII. Meiosis was induced at the indicated temperature. n≥30. (D) Time-lapse images of WT and skp1-a7 cells expressing GFP-Atb2 (α2-tubulin) from the MI onset until anaphase/telophase of MII. In the skp1-a7 cell, the spindle abnormally bent at 25.5 min before full elongation, and collapsed at 27 min. Note that the WT spindle never bent before reaching the cell cortex. The crossing spindles in MII are shown at 51∼67.5 min. Bars, 5 µm.