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. 2012 Jan 23;7(1):e30622. doi: 10.1371/journal.pone.0030622

Figure 3. The chromosome entanglement is made through meiotic recombination.

Figure 3

(A) Chromosome segregation at MI in WT and skp1-a7 cells was monitored in the rec8Δ and rec12Δ background. Note that the spindle was not bent in the skp1-a7 rec8Δ and skp1-a7 rec12Δ. GFP-Atb2 (green) and Taz1-2mCherry (red) are shown. (B) Suppression of the crossing-spindle phenotype in skp1-a7 cells by rec12Δ. Frequency of ‘crossing’ spindles in MII at 25°C and 33°C. n≥30. Error bars, SEM. (C) The spindle was straight in skp1-a7 rec12Δ moa1Δ zygotes. Note that in rec12Δ moa1Δ cells, sister-chromatids are joint until metaphase I and then the sister-chromatids are segregated because of the kinetochore bipolarity. (D) Rhp51-ECFP foci persisted until MI in the skp1-a7 mutant. GFP-Atb2 (green) and Rhp51-ECFP (red) are shown. (E) Rad22-mCherry foci also persisted until MI in the skp1-a7 mutant. Bars, 5 µm.