Figure 4. Effect of myc RNAi on cell cycling activity and cell type distribution.

Cell cycling activity of epithelial cells (A) and 1 s+2 s stem cells (B) were obtained in control and treated animals by continuous incubation with BrdU, followed by maceration of ten animals at the indicated time points and fluorescence immunostaining. Data represent the average of three different experiments. In (C) and (D) the distribution of different cell types was assayed after 4 d and 9 d of myc RNAi, respectively. Five test animals were randomly selected from a pool of twenty five treated animals, and macerated for cell counting. The data represent the average of five independent RNAi experiments. Bars indicate standard errors. 1 s+2 s = single and pairs of interstitial cells; nematoblasts = nests of 4 s−16 s proliferating and differentiating nematoblasts; EPI = epithelial cells.