Figure 5. Hymyc1 RNAi induces an increase in the nematocyte content of battery cells.

(A) At time 4 d and 9 d of treatment with the indicated siRNA, animals were fixed and the number of nematocytes embedded in the battery cells, observed under the same focus plane, was scored. Data represent the average of measures from ten animals ± standard deviation. (B) Representative battery cells imaged from normal (left), myc-siRNA (middle) and luc-siRNA (right) treated animals, on whole mounts. Hand drawn black lines indicate the battery cell membranes. Hymyc1 downregulation induces nematocyte differentiation and accumulation into battery cells. Scale bar: 20 µm.