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. 2012 Jan 23;7(1):e30612. doi: 10.1371/journal.pone.0030612

Figure 1. NLRP12 deficiency does not influence the development of acute allergic airway inflammation.

Figure 1

A) Schematic illustrating OVA mediated acute allergic airway inflammation with an alum adjuvant. B) Mice immunized with OVA/alum and challenged with OVA (OVA/OVA) demonstrated a significant increase in BALF cellularity compared to wild type and Nlrp12−/− mice immunized with OVA/alum but challenged with saline (OVA/Saline). C) Differential staining of the BALF cellularity revealed that mice immunized and challenged with OVA demonstrated a significant increase in airway leukocyte populations, which were predominately eosinophilic. D) A significant increase in local (BALF) and systemic (serum) IL-13 was observed in all OVA immunized and challenged animals regardless of genotype. OVA/Saline, n = 6; Nlrp12−/−, n = 12; wild type, n = 15. E) A significant increase in OVA specific IgE was detected in serum collected from both wild type and Nlrp12−/− mice following OVA immunization and challenge. F) Central airway resistance (Rn) and tissue damping (G) in response to methacholine (MCh) was evaluated in wild type and Nlrp12−/− mice following OVA challenge. Mock (PBS), n = 4; Wild Type, n = 8; Nlrp12−/−, n = 5.