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. 2012 Jan 23;7(1):e30653. doi: 10.1371/journal.pone.0030653

Figure 7. GBV-C NS3/4A/4B expression does not decrease CD4 or CXCR4 expression.

Figure 7

Jurkat cells were transfected with 1 µg GFP in pCDNA3.1/Zeo+ and either 6 µg NS3/4A/4B expression plasmid (red line) or CAT expression plasmid (blue line) and scrambled anti-CD4 siRNA. Equal numbers of viable cells were labeled with anti-CD4 PE-Cy7 and anti-CXCR4-APC and analyzed by FACS on days 3 (A and B), 4 (C and D), and 5 (E and F) post transfection. Cells were gated for GFP expression and then analyzed for CD4 and CXCR4 expression. As a control to demonstrate reduction in receptor expression, cells were transfected with 1 µg GFP in pCDNA3.1/Zeo+ and 6 µg CAT expression plasmid plus anti-CD4 siRNA (A,C,E: gray shaded area, B,D,F: black line).