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. 2012 Jan 23;7(1):e30817. doi: 10.1371/journal.pone.0030817

Figure 4. Stable transgenic Lotus japonicus plant expressing gLjSYMREM1:YFP.

Figure 4

A genomic construct consisting of the LjSYMREM1 native promoter (pLjSYMREM1) and the LjSYMREM1 gene was fused to YFP (gLjSYMREM1:YFP). Roots were inoculated with M. loti MAFF303099-DsRed and three week old nodules of stable transgenic T2 plants were analyzed as 150 µm thick sections. Nodule of a transgenic Lotus plant shows strong YFP-fluorescence in infected cells that co-localizes with M. loti (A–D). Close-ups of infected (yellow) and uninfected (ui; no fluorescence) cells at the periphery of the nodule cortex (E–H). Vacuoles (V) are visible in the center of infected cells. Remnant trans-cellular infection threads lacking gLjSYMREM1:YFP protein are marked with an asterisk. Cross-section of an untransformed control nodule shows no fluorescence (I–L). Bars indicate 100 µm (A–D, I–L), 20 µm (E–G) and 10 µm (H). BF = bright-field.