Figure 6. Rescue of the cuticular phenotype of gsb mutant embryos by gsb-Prd and gsb-Pax3 transgenes.

Ventral view of cuticle preparations of wild-type (ry⁵⁰⁶; A) and homozygous Df(2R)IIX62 embryos without (B) and with one copy of the gsb-Prd (C) or gsb-Pax3 transgene (D) are shown under dark-field illumination (anterior is up). Wild-type and gsb mutant embryos were collected from the Df(2R)IIX62/SM1 stock, while gsb mutant embryos carrying one copy of the transgenes were collected from crosses between Df(2R)IIX62/SM1; gsb-Prd or Df(2R)IIX62/SM1; gsb-Pax3 males and Df(2R)IIX62/SM1 females. gsb mutants were distinguished from wild type by the presence of the zip phenotype, a deformed head structure resulting from the deletion of the zip gene, which is uncovered by Df(2R)IIX62 [30]. Scale bar: 50 um.