Figure 7. Rescue of the CNS phenotype of gsb mutant embryos by gsb-Prd and gsb-Pax3 transgenes.

Patterns of longitudinal and commissural axons in the CNS of wild-type (ry⁵⁰⁶; A) and Df(2R)IIX62/gsb525 embryos without (B) and with one copy of the gsb-Prd (C) or gsb-Pax3 transgene (D). Embryos at stage 15 were collected from crosses between Df(2R)IIX62/CyO, hb-LacZ; gsb-Prd/+ or Df(2R)IIX62/CyO, hb-LacZ; gsb-Pax3/+ males and gsb525/CyO, hb-LacZ females, and double stained with rabbit antiserum against ß-galactosidase and monoclonal antibody BP102. One quarter of the embryos did not stain for ß-galactosidase as expected. Half of these embryos have missing or reduced posterior commissures as expected for gsb mutants, the other half displays fully rescued commissural patterns as in wild-type embryos. Scale bar: 10 um.