Skip to main content
. 2012 Jan 23;7(1):e30895. doi: 10.1371/journal.pone.0030895

Figure 4. Genomic and functional conservation of the 57 bp CYP3A promoter region in primates.

Figure 4

(A) Representation of the evolution of the 57 bp region. Deletions are shown as stretch of hyphens, with the widest one corresponding to the deletion of the entire 57 bp region. 1a–b and 2 indicate the two alternative scenarios of the 57 bp deletion. “CYP” has been removed from gene names to improve legibility. A 7 bp fragment present only in all CYP3A43 genes has been removed for clarity and it position in the human CYP3A43 gene is indicated by an arrow. (B) The effect of galago CYP3A91- and CYP3A92-derived 57 bp regions on the human CYP3A5 promoter activity in MDCK.2 cells. Data are expressed as mean values (±SEM) of five independent experiments conducted as triplicates. Promoter-driven firefly luciferase activities in the individual wells were normalized using activities of the co-transfected renilla luciferase driven by a constitutive promoter. Statistically significant differences are indicated by asterisks *p<0.05, ***p<0.001.