Table 3(a).
Esq/hq(XDH) | Esq/hq(XO) | Difference a | location | |
---|---|---|---|---|
increasing the Esq/hq Difference between XDH and XO | ||||
Lys433 | 40 | 152 | −112 | loop b |
Glu263 | −177 | −104 | −73 | |
Asp429 | −137 | −66 | −71 | loop b |
Asp430 | −108 | −46 | −62 | loop b |
Asp360 | −236 | −190 | −46 | |
Arg426 | 88 | 125 | −37 | loop b |
Asn71 | −14 | 15 | −29 | |
Glu267 | −32 | −10 | −22 | |
FeS-IIc | −151 | −129 | −22 | |
Gln144 | −14 | 7 | −21 | |
decreasing the Esq/hq Difference between XDH and XO | ||||
Lys422 | 261 | 155 | 106 | loop b |
P (proximal)d | 14 | −81 | 95 | |
Lys1228 | 127 | 80 | 47 | |
Lys271 | 53 | 22 | 31 | |
Lys343 | 90 | 67 | 23 | |
Ala338 | 30 | 10 | 20 |
Esq/hq(XDH) − Esq/hq(XO). A negative value here indicates an increased difference between Esq/hq in the two enzymes, while a positive value indicates a decreased difference.
The loop region (Gln423–Lys433) close to the flavin ring 5.
The iron-sulfur cluster proximal to the flavin ring.
A phosphate titratable site of the FAD diphosphate region proximal to the flavin ring.