Table 5(a).
Esq/hq(XDH) | Esq/hq(XDH with NAD+) | Difference a | |
---|---|---|---|
NAD+ | 358 | 358 | |
Lys422 | 261 | 323 | 62 |
P (NAD+, proximal)b | 36 | 36 | |
Arg394 | 60 | 90 | 30 |
Lys343 | 90 | 114 | 24 |
Esq/hq(XDH with NAD+) − Esq/hq(XDH). A positive value here indicates an increased difference between Esq/hq in the two enzymes, while a negative value indicates a decreased difference.
A phosphate titratable site of the NAD+ diphosphate region proximal to the nicotinamide ring.