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Nonlinear regression fitting of Michealis-Menten plots of coupled enzyme reactions in picoliter volume reaction device (ν) and plate reader (λ). The Km and Vmax for the reaction device were found to be 1.65 ± 0.17 mM and 67 ± 1.5 μM min-1 respectively. For comparison, the Km and Vmax for the coupled enzyme reactions carried out in a plate reader (100 μL volume) were found to be 0.75 ± 0.04 mM and 25 ± 0.3 μM min-1 respectively. The inset expands the low glucose concentration region.
