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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2011 Sep 17;52(2):474–484. doi: 10.1016/j.yjmcc.2011.09.008

Figure 1. Adiponectin is expressed in and secreted from VSMC.

Figure 1

(A) Adiponectin gene expression is detected in wild-type mouse aorta and soleus muscle. Mouse aortas were subjected to brief enzymatic digestion to facilitate complete adventitia removal prior to RNA isolation. (B-C) Adiponectin oligomers are detected in both non-transfected (B) and adiponectin plasmid-transfected hCASMC (B-C) lysates and media. Lysates and serum-free cell culture media (TCA precipitated) from non-transfected hCASMC (B), as well as from hCASMC transfected with pcDNA3 (Ctrl) (B) or adiponectin plasmid (B-C) for 48h were subjected to non-denaturing and non-reducing western blotting analysis for adiponectin oligomers expression. In (B), long and short ECL exposures, as well as Ponceau staining (loading control) of the same blot are shown. 60 μg protein per lane was loaded for the non-transfected hCASMC lysates, and 40 ug protein per lane was loaded for the pcDNA3 (Ctrl) (B) or adiponectin plasmid (B-C) transfected lysates. Media from two 10 cm dishes of non-transfected hCASMC, as well as from two 6 cm dishes of pcDNA3 (Ctrl) (B) or adiponectin plasmid (B-C) transfected hCASMC were collected and subjected to TCA precipitation.