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. Author manuscript; available in PMC: 2013 Jan 17.
Published in final edited form as: Biochemistry. 2012 Jan 6;51(2):706–714. doi: 10.1021/bi201708z

Figure 4.

Figure 4

Sgf29p is required for TBP recruitment (and hence transcription) to the ADH1 and PHO84 promoters. (A and B) Analysis of the recruitment of TBP to the ADH1 and PHO84 core promoters in Δsgf29. Yeast strains were grown in YPD (yeast extract containing peptone plus 2% dextrose) at 30 °C up to an OD600 of 1.0 prior to crosslinking. Immunoprecipitation was performed as described in Figure 3A. Primer-pairs targeted to the ADH1 and PHO84 core promoters were used for PCR analysis of the immunoprecipitated-DNA samples. (C) Transcription. Total cellular RNA was prepared from the wild type and Δsgf29 strains, and mRNA levels from the ADH1, PHO84 and RPS5 genes were quantitated by primer-extension analysis.