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NMR titration of the histidine side chain. A) pH 7.0 snapshot of a long-range 1H-15N HSQC experiment to detect the pH dependent protonation of the two histidine side chains in the R24H Pitx2 homeodomain mutant. B) Chemical shift titration curves of the 15N chemical shifts of the histidine Nδ1 side chain resonances with fits to equation 1. Filled triangles correspond to H7 and filled circles to H24.
