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. Author manuscript; available in PMC: 2013 Jan 17.
Published in final edited form as: Biochemistry. 2012 Jan 6;51(2):665–676. doi: 10.1021/bi201639x

Figure 4.

Figure 4

S2 generalized order parameters for the wild-type (●) and R24H mutant (■) Pitx2 homeodomain. The N-terminal arm is highly flexible in the absence of a DNA binding partner. The three helices exhibit average S2 values of ~0.85 suggesting only a limited degree of internal motion. The connecting loop regions L1 and L2 generally have lower S2 values indicative of increased ps to ns internal motion in these regions. Similar to the N-terminus, the C-terminus is highly mobile (including the 6 amino acid artificial tale – data not shown). The vertical dashed line indicates the position of residue 24.