Table 2.
Results of ITC measurements on Pitx2 homeodomain/DNA complexes
K(M−1) | Kd (nM) | ΔG° (kcal/mol) | ΔH° (kcal/mol) | −TΔS° (kcal/mol) | |
---|---|---|---|---|---|
Pitx2HD wt / TAATCC | 1.51(±0.29) × 107 | 66 | −9.84±0.27 | −7.91±0.17 | −1.93±0.10 |
Pitx2HD R24H / TAATCC | 3.79(±0.37) ×107 | 26 | −10.32±0.12 | −7.53±0.30 | −2.79±0.36 |