In situ hybridization of (A) miR-223, (B) miR-21, (C) miR-29a, and (D) miR-126 (magenta), with simultaneous immunofluorescent detection of CK19 to detect cholangiocytes (green). In (A), the inset shows a higher magnification view of infiltrating monocytes (arrows). In (B) and (C), the insets include higher magnification views of bile ductules. Liver sections from the 8 days post saline (control) or RRV injection were hybridized with locked nucleic acid miRNA probes and a rabbit polyclonal CK19 antiserum.