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. Author manuscript; available in PMC: 2013 Jan 20.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Dec 27;417(3):1058–1062. doi: 10.1016/j.bbrc.2011.12.095

Fig. 3.

Fig. 3

Phosphorylation of positive regulatory tyrosine of nuclear Lck. Cytosolic and nuclear fractions were isolated from LSTRA (A) and T-REx-293/Y505FLck (B) cells as described for Fig. 2. Normalized total proteins were immunoprecipitated with anti-Lck antibody, followed by immunoblotting with antibody specific for Tyr 394-phosphorylated Lck. The membrane was stripped and then reblotted for total Lck using anti-Lck (A) or anti-Myc tag (B) antibody. The arrowheads mark the positions of immunoglobulin heavy chain (IgH).