Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Feb 1.

Published in final edited form as: Brain Behav Immun. 2011 Sep 21;26(2):218–227. doi: 10.1016/j.bbi.2011.09.006

Restraint-10 mice were exposed to 50 or 200 cGy of gamma radiation (44.5 ± 0.1 cGy/min) as indicated. qPCR was used to quantify mRNAs from blood and un-perfused and perfused whole brains as indicated 8 h post irradiation. Results are expressed as relative fold change in mRNA expression (ΔmRNA), means ± s.e.m.; n = 4–6. (A) IL-1β: main effect of dose (P < 0.001); P < 0.05, 200 cGy v. sham IR, 50 cGy (3.312 ± 0.468 v. 1.000 ± 0.182, 0.711 ± 0.254, respectively). (B) 8 h IL-1RA: main effect of dose (P < 0.001); P < 0.05, 200 cGy v. sham IR, 50 cGy (3.335 ± 0.446 v. 1.000 ± 0.146, 0.690 ± 0.273, respectively). (C) IL-1β: main effect of dose (P = 0.006), dose-perfusion interaction (P = 0.002); P < 0.05, 200 cGy (unperfused) v. sham IR (unperfused), 50 cGy (unperfused), sham IR, 50 cGy (2.555 ± 0.267 v. 1.000 ± 0.570, 0.557 ± 0.182, 0.755 ± 0.407, 1.052 ± 0.189, respectively). (D) IL-1RA: main effect of dose (P < 0.001); P < 0.05, 200 cGy (unperfused) v. sham IR (unperfused), 50 cGy (unperfused), sham IR, 50 cGy (3.013 ± 0.255 v. 1.000 ± 0.531, 0.570 ± 0.244, 0.982 ± 0.488, 0.935 ± 0.258, respectively); P = 0.009, 200 cGy v. 50 cGy (unperfused) (1.961 ± 0.267 v. 0.570 ± 0.244). Bars without a common superscript are different (P < 0.05).

Restraint-10 mice were exposed to 50 or 200 cGy of gamma radiation (44.5 ± 0.1 cGy/min) as indicated. qPCR was used to quantify mRNAs from blood and un-perfused and perfused whole brains as indicated 8 h post irradiation. Results are expressed as relative fold change in mRNA expression (ΔmRNA), means ± s.e.m.; n = 4–6. (A) IL-1β: main effect of dose (P < 0.001); P < 0.05, 200 cGy v. sham IR, 50 cGy (3.312 ± 0.468 v. 1.000 ± 0.182, 0.711 ± 0.254, respectively). (B) 8 h IL-1RA: main effect of dose (P < 0.001); P < 0.05, 200 cGy v. sham IR, 50 cGy (3.335 ± 0.446 v. 1.000 ± 0.146, 0.690 ± 0.273, respectively). (C) IL-1β: main effect of dose (P = 0.006), dose-perfusion interaction (P = 0.002); P < 0.05, 200 cGy (unperfused) v. sham IR (unperfused), 50 cGy (unperfused), sham IR, 50 cGy (2.555 ± 0.267 v. 1.000 ± 0.570, 0.557 ± 0.182, 0.755 ± 0.407, 1.052 ± 0.189, respectively). (D) IL-1RA: main effect of dose (P < 0.001); P < 0.05, 200 cGy (unperfused) v. sham IR (unperfused), 50 cGy (unperfused), sham IR, 50 cGy (3.013 ± 0.255 v. 1.000 ± 0.531, 0.570 ± 0.244, 0.982 ± 0.488, 0.935 ± 0.258, respectively); P = 0.009, 200 cGy v. 50 cGy (unperfused) (1.961 ± 0.267 v. 0.570 ± 0.244). Bars without a common superscript are different (P < 0.05).