Immunofluorescent localization of GPR30 (A,B), mPRα (C–E) and olfactory G protein (Golf, F) in teleost oocytes, ovarian follicles and sperm using specific GPR30, mPRα and Golf antibodies. A. GPR30 localization on late-stage vitellogenic zebrafish oocytes in ovarian tissue cryosections. Scale bar=100 µm. B. Co-staining with DAPI of nuclear DNA of zebrafish follicular cells. Reproduced from Pang and Thomas, Dev. Biol. 342 (2010) 194–206 [159], Figure 2, with permission. C. mPRα localization on zebrafish oocytes using a mPRα antibody. Insert, high magnification showing mPRα on oocyte membrane. Reproduced from Hanna et al. J. Endocrinol. 190 (2006) 247–260 [82], Figure 2, with permission. D. mPRα localization in Atlantic croaker granulosa and theca cell cultures using the mPRα antibody and DAPI nuclear staining. Scale bar= 10 µm. From Dressing et al., Endocrinology 151 (2010) 5916–5926 [44], Figure 1, with permission. E, F. Immunocytochemistry of Atlantic croaker sperm using mPRα (E) and Golf (F) antibodies and DAPI nuclear staining. Scale bar=5µm. From Tubbs and Thomas, Endocrinology 150 (2009) 473–484 [239], Figure 7, with permission.