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Competitive binding of the phage-VHH. A 96-well plate was coated with 3-PBA hapten-BSA conjugate at 0.4 μg/mL. Dilutions of 3-PBA in PBS was mixed with a 1/2500 dilution of the phage-VHH in PBS and 100 μl of the mixture was added into wells. The bound phage-VHHs were detected by adding 100 μl of mouse anti-M13 phage MAb-HRP diluted in PBS at 1/5000.
