Table I.

Effect of estradiol (1 nM) or ER agonists (10 nM) on sodium currents and action potentials in isolated GnRH neurons from young female mice.

Property	Young Control		Young Estradiol		Young DPN		Young PPT
	AM	PM	AM	PM	AM	PM	AM	PM
Sodium Currents:
Amplitude (pA/pF)	−532.58±28.0 n = 25	−475.18±24.0 n = 24	−405.57±36.5a n = 15	−551.0±13.0b n = 13	−357.10±34.6a n = 12	−503.66±44.4b n = 10	−443.11±55.5 n = 10	−556.60±45.9 n = 10
Rise slope (V/ms) (maximum)	−21.43±1.8 n = 21	−19.50±1.9 n = 22	−14.30±1.6a n = 11	−20.48±2.9 n = 10	−15.12±2.4a n = 12	−21.40±3.11 n = 10	−22.30±3.9 n = 7	−30.61±6.9 n = 7
Decay slope (V/ms) (maximum)	12.92±1.1 n = 21	12.49±1.0 n = 22	8.59±1.0a n = 11	13.27±1.9 n = 10	9.42±1.2a n = 12	13.63±1.9 n = 10	12.26±2.3 n = 7	19.11±4.3 n = 7
Width (1/2 max; ms)	0.47±0.006 n = 21	0.49±0.02 n = 22	0.49±0.02 n = 11	0.48±0.02 n = 10	0.50±0.01a n = 12	0.44±0.007b,c n = 10	0.48±0.01 n = 7	0.47±0.008 n = 7
Action Potentials:
Amplitude (mV)	128.6±1.07 n = 17	126.8±2.39 n = 14	125.4±3.42 n = 8	129.1±1.17 n = 9	126.2±2.0 n = 9	126.5±1.67 n = 7	131.9±0.9 n = 4	125.4±1.5 n = 3
Width (1/2 max; ms)	1.55±0.07 n = 17	1.53±0.07 n = 14	1.64±0.13 n = 8	1.50±0.07 n = 9	1.72±0.12d n = 9	1.46±0.05 n = 7	1.65±0.08 n = 4	1.53±0.15 n = 3
AHP Amplitude (mV)	−4.65±0.55 n = 17	−4.48±0.54 n = 14	−4.35±0.44 n = 8	−3.82±0.46 n = 9	−3.82±1.05 n = 9	−3.20±0.82 n = 7	−4.46±0.09 n = 4	−4.30±0.27 n = 3
DAP Amplitude (mV)	4.68±0.26 n = 23	4.66±0.23 n = 16	3.51±0.41a n = 11	4.07±0.53 n = 10	4.79±0.68 n = 9	3.51±0.51c n = 7	6.27±0.62e n = 4	4.19±0.28 n = 3

^aSignificantly different from AM control (p<0.05).

^bSignificantly different from same-treatment AM (p<0.05).

^cSignificantly different from PM control (p<0.05).

^dSignificantly different from same-treatment PM (p<0.05).

^eSignificantly larger than AM control or estradiol DAP (p<0.05).