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. 2011 Aug 11;29(2):352–361. doi: 10.1007/s11095-011-0545-z

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© The Author(s) 2011

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Fig. 8 — Cellular uptake of PEGylated polyplexes. (a) Mean fluorescence intensity (MFI) of H1299 cells determined 4 h after transfection, corrected for protein content. Polyplexes were prepared using polymers with increasing PEG content and fluorescently labeled siRNA. Lipofectamine 2000 was used as control. Results are shown as mean ± SD for n = 2–3. Statistically significant differences versus polyplexes without PEG are denoted by * (p < 0.05) or *** (p < 0.001). (b) Fluorescent microscopy pictures of H1299 treated cells. Nuclei are stained blue (DAPI), siRNA appears as green (Alexa-488).