Table 2.
Presence and activity of Rpi genes in transgenic Desiree plants
| l (# of) plant | PCR | Pi inoculation | Effector agro-infiltration | ||||||
|---|---|---|---|---|---|---|---|---|---|
| nptII | sto1 | vnt1.1 | blb3 | EC1 | Pic99189 | IpiO1 | Avrvnt1 | Avr2 | |
| Regenerants (28) | |||||||||
| 23 | + | + | + | + | R | R | HR | HR | HR |
| 5 | + | − | − | − | S | S | − | − | − |
| Control | |||||||||
| Desiree:Rpi-sto1 a | + | + | − | − | R | S | HR | − | − |
| Desiree:Rpi-vnt1 | + | − | + | − | S | R | − | HR | − |
| Desiree:Rpi-blb3 | + | − | − | + | R | S | − | − | HR |
| Desiree | − | − | − | − | S | S | − | − | − |
The presence of the Rpi genes was determined by PCR. Biological activity of the Rpi genes was determined by Pi inoculation in detached leaf assays or by agro-infiltration using Avr effectors matching the introduced Rpi genes
HR Hypersensitive response
R Resistant
S Susceptible
“−” in PCR means no PCR product using gene specific primer pairs, and “−” in agro-infiltration means no HR symptom
aDesiree:Rpi-sto1 stands for one cv. Desiree transformant harbouring Rpi-sto1