Fig. 2.

Urinary sediments excreted 2 days after 50 μg/kg DT administration. A A urine sample was spread onto a glass slide, fixed with methanol and stained with Giemsa solution. B Another urine sample on the slide was fixed with paraformaldehyde and incubated with rabbit anti-AQP7 antibody followed by an AlexaFluor594-conjugated anti-rabbit IgG antibody (red in b and c). DAPI was used to detect nuclei (blue in a and c). We can find cell debris expressing AQP7 along with the intact cells in urine